Owners Julia and Steve have announced their departure via Facebook.
The bar first opened in 2019 and has since hosted hundreds of live music events, featuring artists such as Albert Lee, The Bluetones, Chris Helme and Nigel Clarke.
Over the years, it has built a reputation as a live music venue, receiving two awards in recognition of its contribution to the local scene.
Former owners Julia and Steve reflected on the journey, thanking staff, musicians and customers for their support.
"Thank you to all the brilliant staff members who've ever worked for us since we opened.
"The team make the bar, and you all certainly did us proud! Huge thanks too to all the amazing musicians who have graced our stage, and squeezed into the Little Mess corner".
The couple made the decision to step down as owners to travel around musical states in America.
The pair continued:
"We could never have imagined that when we had the idea of creating a new quirky bar (off the beaten track), that you'd all welcome us with wide, open arms and make the bar such a success.
"Thank you from the bottom of our hearts.
"As much as we absolutely love The Mess Room, it's now time for us to take some time to enjoy life a little, and place ourselves at the other side of the bar for a while"
Future events and bookings at The Mess Room will continue as planned under the new ownership.
Steve will remain involved in the music scene as a freelance sound engineer, while Julia and Steve both intend to explore new ventures, starting with a trip to the United States.

Pop-up pumpkin patch and monster trail coming to Skipton
Motorcyclist dies following collision on A629 near Skipton
Louis Theroux makes special visit to Martin House children's hospice
Skipton football matches raise £2,100 for Manorlands Hospice
Stump Cross Caverns named finalist in family business awards
Keighley Healthy Living celebrates 25 years of growth
Dunelm Keighley partner with Airedale Hospital for Christmas appeal
Popular Big Skipton Quiz returns to raise funds for children’s charity
Leeds Bradford Airport strikes to 'escalate' over half term
Yorkshire Water sewer capacity threatens new homes and funding, says councillor
Hellifield businessman raises over £1,400 with 50-mile challenge
Growing pressure on North Yorkshire farmers, reports warn
Elsworth at The Mill named finalist in prestigious Yorkshire food awards
Cracoe farming content creator set to be part of Yorkshire Farming Conference
Skipton Business Awards raises more than £1,600 for cancer charity
Scarborough’s Alpamare waterpark set to be sold with 99-year car park lease
Martin House opens new memorial garden for bereaved families
More than £80,000 raised at Sue Ryder Starlight Hike
Mayor joins India trade mission as leaders target £170m boost for Yorkshire
Terrifying Halloween Scare Train returning to Keighley railway


